LAUSR

Cellular membranes consist of a large variety of lipids and proteins, with a composition that generally differs between the two leaflets of the same bilayer. One consequence of this asymmetry is thought to be the emergence of differential stress, i.e., a mismatch in the lateral tension of the two leaflets. This can affect a membrane’s mechanical properties; for instance, it can increase the bending rigidity once the differential stress exceeds a critical threshold. Using coarse-grained molecular dynamics simulations based on the MARTINI model, we show that this effect arises due to the formation of more highly ordered domains in the compressed leaflet. The threshold asymmetry increases with temperature, indicating that the transition to a stiffened regime might be restricted to a limited temperature range above the gel transition. We also show that stiffening occurs more readily for larger membranes with smaller typical curvatures, suggesting that the stiffening transition is easier to observe experimentally than in the small-scale systems accessible to simulation.