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Expression analysis of chitinase upon challenge inoculation to Alternaria wounding and defense inducers in Brassica juncea

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Highlights • Expression of chitinase gene was studied by RT-PCR in response to Alternaria brassicae.• Chitinase gene is induced by Alternaria, wounding and by JA and not by SA. It… Click to show full abstract

Highlights • Expression of chitinase gene was studied by RT-PCR in response to Alternaria brassicae.• Chitinase gene is induced by Alternaria, wounding and by JA and not by SA. It shows the tissue specificity of the gene.• Pathogen-inducible 2.5 kb chitinase class IV promoter was isolated from B. juncea by Genome Walking.• Induction pattern of chitinase gene is also reflected in promoter validation studied in transgenic Arabidopsis leaf.• This will help in using this promoter discretely in developing fungus resistant transgenic plants.

Keywords: expression analysis; alternaria wounding; chitinase gene; chitinase

Journal Title: Biotechnology Reports
Year Published: 2017

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