Highlights • Triosephosphate isomerases from different species have different numbers of rare codons for E. coli.• They only have rare codons for Arg, which distribute differently in the corresponding sequence.•… Click to show full abstract
Highlights • Triosephosphate isomerases from different species have different numbers of rare codons for E. coli.• They only have rare codons for Arg, which distribute differently in the corresponding sequence.• Protein expression in E. coli strain CP (DE3)-RIL increases with the number of rare codons for Arg.
               
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