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Highlights • Biologically active rhBMP-4 was produced in a prokaryotic host as inclusion bodies.• Different refolding recipes were tested for optimal dimerization yield.• One-step purification of dimer with cation-exchange membrane… Click to show full abstract
Highlights • Biologically active rhBMP-4 was produced in a prokaryotic host as inclusion bodies.• Different refolding recipes were tested for optimal dimerization yield.• One-step purification of dimer with cation-exchange membrane chromatography.• The product induces trophoblast differentiation in induced pluripotent stem cells.• Comparison between commercial rhBMP-4 from cell culture and product from E. coli.
Keywords:
inclusion bodies;
renaturation biologically;
biologically active;
active human;
solubilization renaturation
Journal Title: Biotechnology Reports
Year Published: 2018
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Journal Title: Biotechnology Reports
Year Published: 2018
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!