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A multiplex qPCR TaqMan-assay to detect fungal antagonism between Trichoderma atroviride (Hypocreaceae) and Botrytis cinerea (Sclerotiniaceae) in blackberry fruits using a de novo tef1-α- and an IGS-sequence based probes

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Highlights • Quantitative polymerase chain reactions specifically detect Trichoderma atroviride.• The method uses a probe based on the tef-1α for the detection.• The method simultaneously detects T. atroviride and Botrytis… Click to show full abstract

Highlights • Quantitative polymerase chain reactions specifically detect Trichoderma atroviride.• The method uses a probe based on the tef-1α for the detection.• The method simultaneously detects T. atroviride and Botrytis cinerea in blackberries.

Keywords: botrytis cinerea; qpcr taqman; trichoderma atroviride; multiplex qpcr

Journal Title: Biotechnology Reports
Year Published: 2020

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