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Investigating thermal stability based on the structural changes of lactase enzyme by several orthogonal methods

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Highlights • The primary and secondary structure of lactase was not changed by thermal stress.• The glycan structure of lactase showed significant alterations after heat stress.• Changes in the glycosylation… Click to show full abstract

Highlights • The primary and secondary structure of lactase was not changed by thermal stress.• The glycan structure of lactase showed significant alterations after heat stress.• Changes in the glycosylation structure affected physico-chemical properties of lactase.

Keywords: structural changes; thermal stability; changes lactase; based structural; stability based; investigating thermal

Journal Title: Biotechnology Reports
Year Published: 2021

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