Photo from wikipedia
Highlights • Stable secondary RNA structure 3′ of the start codon inhibits ymNeonGreen expression.• Removing secondary RNA structure increased expression in S. cerevisiae and E. coli.• Expression was increased 2-fold… Click to show full abstract
Highlights • Stable secondary RNA structure 3′ of the start codon inhibits ymNeonGreen expression.• Removing secondary RNA structure increased expression in S. cerevisiae and E. coli.• Expression was increased 2-fold in S. cerevisiae.• Expression was increased 3.8-fold in E. coli.• Increased expression in S. cerevisiae was promoter independent.
Keywords:
fluorescent reporter;
expression;
start codon;
increased expression;
structure;
expression fluorescent
Journal Title: Biotechnology Reports
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!
Journal Title: Biotechnology Reports
Year Published: 2022
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!