LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Qualitation and quantification of water soluble non-starch polysaccharides from Pseudostellaria heterophylla in China using saccharide mapping and multiple chromatographic methods.

Photo from archive.org

Qualitative and quantitative analysis of non-starch polysaccharide (NSP) from Pseudostellaria heterophylla in China (Guizhou, Anhui and Fujian) were performed using high performance size exclusion chromatography coupled with multi-angle laser light… Click to show full abstract

Qualitative and quantitative analysis of non-starch polysaccharide (NSP) from Pseudostellaria heterophylla in China (Guizhou, Anhui and Fujian) were performed using high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector (HPSEC-MALLS-RID), saccharide mapping based on polysaccharide analysis by using carbohydrate gel electrophoresis (PACE) and high performance thin layer chromatography (HPTLC). Results showed that PACE and HPTLC fingerprints of enzymatic hydrolysates of NSP were similar. Moreover, the results indicated that 1,4-β-d-Galp, 1,5-α-Araf, 1,4-α-d-GalAp and 1,4-β-d-Glcp linkages existed in NSP. Based on the results of HPSEC-MALLS/RI, the Mw, Rg, and contents of NSP in P. heterophylla were ranging from 4.37 × 104 to 1.70 × 105 Da, 46.4 to 65.9 nm, and 0.43% to 1.41%, respectively. There were no significant difference (p>0.05) observed among the three main producing areas, which suggested that polysaccharides could be used as marker for quality control of P. heterophylla.

Keywords: non starch; saccharide mapping; pseudostellaria heterophylla; heterophylla; heterophylla china

Journal Title: Carbohydrate polymers
Year Published: 2018

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.