LAUSR

The sea buckthorn (Hippophae rhamnoides L.) berries are rich in various bioactive components and widely used as fruit and traditional medicine. In this study, a novel heteropolysaccharide fraction (SP0.1-1) was isolated from Sea buckthorn berries. SP0.1-1 is composed of mannose, glucose, galactose, and arabinose in the molar ratio of 1:2.3:1.9:11.2 with a core structure containing 1,4-linked-α-d-Glcp, 1,4,6-linked-α-d-Glcp and 1,4-linked-α-d-Manp residues as the backbone. And the side-chains comprised of 1,3,5-linked-α-l-Araf, 1,5-linked-α-l-Araf, terminal α-Araf and 1,4-linked-β-d-Galp. Furthermore, a diet supplemented with SP0.1-1 extended the mean lifespan, enhanced antioxidant enzyme (superoxide dismutase, SOD; glutathione peroxidase, GSH-Px; and catalase, CAT) activities, and decreased the malondialdehyde (MDA) level and hydrogen peroxide (H2O2)-induced mortality rate in fruit flies (Drosophila melanogaster). To summarize, the study's findings will provide evidence for the development of sea buckthorn polysaccharide products.