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SUMMARY At low temperatures, protein degradation by the AAA+ HslUV protease is very slow. New crystal structures reveal that residues in the intermediate domain of the HslU6 unfoldase can plug… Click to show full abstract
SUMMARY At low temperatures, protein degradation by the AAA+ HslUV protease is very slow. New crystal structures reveal that residues in the intermediate domain of the HslU6 unfoldase can plug its axial channel, blocking productive substrate binding and subsequent unfolding, translocation, and degradation by the HslV12 peptidase. Biochemical experiments with wild-type and mutant enzymes support a model in which heat-induced melting of this autoinhibitory plug activates HslUV proteolysis.
Keywords:
aaa hsluv;
hsluv protease;
autoinhibitory plug;
heat;
plug
Journal Title: Cell reports
Year Published: 2021
Link to full text (if available)
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Journal Title: Cell reports
Year Published: 2021
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!