LAUSR

Hexavalent chromium is one of the major detrimental heavy metal pollutants. B. gargarizans tadpoles were treated with different concentrations of Cr6+ (0, 13, 52, 104, 208, and 416 μg Cr6+ L-1) from Gs 2 to Gs 42. The effect of Cr6+ on histopathological alterations and transcript levels of fatty acid metabolism-related genes as well as fatty acids composition and content in liver were examined. Histopathological changes were observed in liver at 52, 104, 208, and 416 μg Cr6+ L-1. Moreover, RT-qPCR analyses showed the downregulated mRNA levels of the genes related to fatty acid synthesis (SCD, MECR, TECR and ELOVL1) and fatty acid β-oxidation (ACOT1, PPT1, HADH and ACAA2) at 416 μg Cr6+ L-1. However, the mRNA expression of fatty acid β-oxidation-related genes (ECHS1, HADHA and ACAA1) were significantly upregulated at 13, 52, 104, 208 and 416 μg Cr6+ L-1. In situ hybridization revealed BSEP was expressed in hepatocyte nucleus and plasma membrane, and HSD17B12 was abundantly expressed in the plasma membrane. The HSD17B12 mRNA levels were significantly upregulated in tadpoles exposed to all Cr6+ treatment groups, while the BSEP mRNA levels were downregulated at 104, 208 and 416 μg Cr6+ L-1 groups compared to control. In addition, an increase in polyunsaturated fatty acids and a decrease in monounsaturated fatty acids were found in 52, 104 and 416 μg Cr6+ L-1 groups. Overall, chronic exposure to Cr6+ may suppress fatty acid synthesis, disturb fatty acid β-oxidation, aggravate disorders of hepatic function and induce hepatic impairment in B. gargarizans tadpoles.