LAUSR

Toxic metals and silver nanoparticles (AgNPs) are of great importance as pollutants and their frequent use increases the risk of exposure to biota, but few studies have described co-toxic effects in aquatic organisms. In fish, the method using early stages of development are interesting parameters to validate ecotoxicological studies, and more recently, the use of mathematical models has substantially increased the efficiency of the method. Post hatching stages of native catfish Rhamdia quelen were exposed to single or combined mixtures of toxic metals (Mn, Pb, Hg or AgNPs) in order to study its effects. Fertilized eggs were exposed for 24, 48, 72, and 96 h, where hatching and survival rates, malformation frequency, and neuromast structure damages were evaluated. The results showed alterations in hatching rate after single and combined exposure to metals, but mixtures showed effects more severe comparatively with the single exposures. A similar result including a time-dependent effect was observed in survival rates and incidence of deformities. Overall, embryos and larvae were sensitive to toxic metals exposure while the mathematical modeling suggested a population reduction size including risk of local extinction.