LAUSR

The complexation mechanism between pharmaceuticals and natural colloids is still uncertain due to the complexity, heterogeneity, and polydispersity of colloids. Therefore, this study investigated the effect of fluorescence properties on the complexation of chloramphenicol (CAP) and carbamazepine (CBZ) to the colloids from Poyang Lake Basin based on the multiple spectroscopic techniques and methods. Three-dimensional excitation-emission matrix fluorescence spectroscopy-parallel factor analysis results illustrated that two humic-like components and two protein-like components of colloids from the rivers and lakes were identified, with the much higher fluorescence intensity of the protein-like substance observed in lake samples. The protein-like substance decreased dramatically with the addition of CAP and CBZ, suggesting its higher binding capacity towards these drugs, especially for CBZ. In addition, the fluorescence quenching titration was proceeded to explore the binding mechanism between the colloids and the pharmaceuticals. Results of synchronous fluorescence spectra and two-dimensional correlation spectroscopy demonstrated that the fluorescence quenching effect occurred preferentially between the protein-like substances and the pharmaceuticals, with the stronger complexation for CBZ. Ryan-Weber model fitting results showed that the stability constant ranged from 4.02 to 5.04 with the higher binding capacity observed for the tryptophan-like substance. Combined, the fluorescence components in aquatic colloids could be significantly impacted the complexation of the pharmaceuticals. This study provides deep insights into the fate and pollution protection of pharmaceuticals.