LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins

Photo from wikipedia

Abstract The importance of immunoglobulin Y (IgY) as a specific antibody equivalent to mammalian immunoglobulin G (IgG) is well recognized. However, production of highly purified IgY is still difficult due… Click to show full abstract

Abstract The importance of immunoglobulin Y (IgY) as a specific antibody equivalent to mammalian immunoglobulin G (IgG) is well recognized. However, production of highly purified IgY is still difficult due to the lack of specific purification methods. In this study, adsorption behaviors of IgY on four mixed-mode resins with functional ligands of 4-mercatoethyl-pyridine (MEP), 2-mercapto-1-methyli-midazole (MMI), 5-aminobenzi-midazole (ABI) and tryptophan-5-aminobenzi-midazole (W-ABI) were evaluated. The results showed that high adsorption ratio were found at pH 6.0–7.0 with little adsorption under acidic conditions. The resin with ABI ligand was then used to separate IgY from immunized chicken serum. An efficient process with IgY purity of 95% and recovery of 90% was developed after optimization of loading and elution pH and injection volume. The biological activity of the purified IgY was fully maintained. These results indicated that mixed-mode chromatography with specially-designed ligands has great potential for the separation of IgY from crude feedstock.

Keywords: adsorption behaviors; purification; chicken serum; mixed mode; mode resins; mode

Journal Title: Chinese Journal of Chemical Engineering
Year Published: 2019

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.