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Abstract Bioenergetic analysis implies that microorganisms can harvest the released energy from metal corrosion for survival via extracellular electron transfer (EET) under carbon-starvation conditions. In this work, we combined carbon… Click to show full abstract
Abstract Bioenergetic analysis implies that microorganisms can harvest the released energy from metal corrosion for survival via extracellular electron transfer (EET) under carbon-starvation conditions. In this work, we combined carbon starvation with CTC/DAPI counterstaining and developed a novel method for detecting EET from metal substrate to the cells. A wild-type nitrate-reducing bacterium (NRB) Bacillus licheniformis was evidenced to be able to take up electrons from the X80 steel substrate for respiration. This study provides reliable support to the bioenergetic theory for microbiologically influenced corrosion (MIC) of carbon steel by NRB.
Journal Title: Corrosion Science
Year Published: 2020
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