LAUSR

Abstract Environmental standardization and stabilization of surface charges of silver nanoparticles (AgNPs) is important in biological systems and interest in bio-interfacial interaction. Different synthesized AgNPs in chemical reduced (AgNO 3 (0.01, 0.1 and 0.5 M); NaBH 4 and Na 3 C 6 H 5 O 7 ) garnered for analysis of physico-chemical charge stabilization by means of different pH (1–13) and ionic interferences (NaCl, Ca(NO 3 ) 2 , Na 2 CO 3 and NaNO 3 ). The uniform sized (size: ∼22 nm) and highly charged (zeta potential: −37.9 mV) AgNPs with uniform dispersion remains unaltered in high ionic interferences. Highest antifungal activity of AgNPs against Candida albicans and moderate activity against Staphylococcus aureus are correlated.