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Abstract We demonstrate a technique which can change the pH inside an NMR tube in steps of ≤0.01 pH unit, during a solution-state NMR experiment. We use a photo-acid co-solute… Click to show full abstract
Abstract We demonstrate a technique which can change the pH inside an NMR tube in steps of ≤0.01 pH unit, during a solution-state NMR experiment. We use a photo-acid co-solute with the actual specimen, and photo-excite the solution by direct fibre-mediated light delivery to the NMR tube. The photo-excitation and NMR measurements can be performed simultaneously. Our method should allow precise variation of the pH in studying protein folding, aggregation and enzyme catalysis processes which often display critical pH dependence.
Keywords:
modulation nmr;
photo;
photo induced;
situ photo;
induced modulation;
precise situ
Journal Title: Chemical Physics Letters
Year Published: 2018
Link to full text (if available)
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Journal Title: Chemical Physics Letters
Year Published: 2018
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!