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OBJECTIVES The aim of this study was to evaluate the plaque-inhibiting effects of oil pulling using 4- day plaque regrowth study model compared to 0.2% chlorhexidine gluconate (CHX) containing mouthrinse. DESIGN The study was an observer-masked, randomized, cross-over design clinical trial, involving 29 volunteers to compare 0.2% CHX and oil pulling therapy in a 4- day plaque regrowth model. After the preparatory period, in which the subjects received professional prophylaxis, the subjects commenced rinsing with their allocated rinsed. On day 5 plaque index (PI), gingival index (GI), stain index (SI), bleeding on probing (BOP) were recorded from the subjects. Each participant underwent a 14- day wash out period and then used the other mouthrinse for four days. RESULTS Oil pulling therapy presented similar inhibitory activity on plaque regrowth compared with CHX (PI = 1.67 ± 0.24, 1.61 ± 0.20, respectively) with less staining (SI = 0.21 ± 0.13, 0.47 ± 0.27, respectively). In addition, GI and BOP was similar in both groups (p > 0.05). CONCLUSION Oil pulling with coconut oil seems to have similar plaque inhibition activity as CHX. In addition it caused less tooth staining than CHX. These findings suggest that oil pulling therapy may be an alternative to CHX rinse.