LAUSR

HighlightsIL‐33 was upregulated in nasal polyps of eosinophilic chronic rhinosinusitis.IL‐33 increase MUC5AC gene and MUC5AC protein in primary human nasal epithelial cell.IL‐33 induces goblet cell hyperplasia in primary human nasal epithelial cell. Abstract We investigated whether IL‐33 is involved in mucus overproduction and goblet cell hyperplasia in eosinophilic chronic rhinosinusitis (ECRS). IL‐33 mRNA was significantly higher in the eosinophilic CRS group than in the non‐eosinophilic CRS group from human nasal polyps. IL‐33 induced MUC5AC mRNA and MUC5AC protein, and also goblet cell hyperplasia at air liquid interface culture in human nasal epithelial cells. In addition to that, IL‐33 induced MUC5B and FOXA3, and reduces FOXJmRNA. In conclusion, our present study demonstrated that the direct evidence of IL‐33 which lead to increase mucin gene and protein expression, as well as goblet cell hyperplasia. This study provides novel insights into the role of IL‐33 on mucus overproduction in eosinophilic inflammation of human airways.