LAUSR

Abstract The Major Histocompatibility Complex (MHC) is a genomic region encoding immune loci that are important and frequently used markers in studies of adaptive genetic variation and disease resistance. Given the primary role of infectious diseases in contributing to global amphibian declines, we characterized the hypervariable exon 2 and flanking introns of the MHC Class II&bgr; chain for 17 species of frogs in the Ranidae, a speciose and cosmopolitan family facing widespread pathogen infections and declines. We find high levels of genetic variation concentrated in the Peptide Binding Region (PBR) of the exon. Ten codons are under positive selection, nine of which are located in the mammal‐defined PBR. We hypothesize that the tenth codon (residue 21) is an amphibian‐specific PBR site that may be important in disease resistance. Trans‐species and trans‐generic polymorphisms are evident from exon‐based genealogies, and co‐phylogenetic analyses between intron, exon and mitochondrial based reconstructions reveal incongruent topologies, likely due to different locus histories. We developed two sets of barcoded adapters that reliably amplify a single and likely functional locus in all screened species using both 454 and Illumina based sequencing methods. These primers provide a resource for multiplexing and directly sequencing hundreds of samples in a single sequencing run, avoiding the labour and chimeric sequences associated with cloning, and enabling MHC population genetic analyses. Although the primers are currently limited to the 17 species we tested, these sequences and protocols provide a useful genetic resource and can serve as a starting point for future disease, adaptation and conservation studies across a range of anuran taxa. HighlightsDeveloped primers that sequence MHC class II&bgr; complete exon 2 for 17 frog species.Intron‐based primers amplify a single locus allowing for population genetic studies.Intron versus exon genealogies highlight incongruences due to historical selection.9/10 positively selected codons match the mammal‐defined peptide‐binding region.454 and Illumina fusion primers can genotype hundreds of individuals in parallel.