Photo from wikipedia
Abstract Two-photon (TP)-based ratiometric fluorescence probe has become an efficient and attractive technique for fluorescence imaging studies of biological events. Therefore, construction of some new type functional TP ratiometric fluorescent… Click to show full abstract
Abstract Two-photon (TP)-based ratiometric fluorescence probe has become an efficient and attractive technique for fluorescence imaging studies of biological events. Therefore, construction of some new type functional TP ratiometric fluorescent dyes has attracted great attention. In this work, we adopted an optically superior TP fluorophore to develop a TP ratiometric semi-rhodamine "switch" fluorescent dye platform (KzRh), and a peroxynitrite anion (ONOO−) fluorescent probe (KzRhONOO) with TP excitation were constructed based-on this platform for detecting ONOO− in living cells and zebrafish, which exhibits a target-modulated ratiometric TP fluorescence response through the semi-rhodamine's close/opene form of the spirocycle. Such a scaffold affords KzRhONOO possesses dual-emission peak separated by 50 nm, rapid response (
Journal Title: Dyes and Pigments
Year Published: 2020
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!