Abstract A new nanopore array/Au electrode was fabricated and applied to the detection of microRNA. The electrode was prepared via alternating current electrodeposition of Au nanowires in the nanopores of… Click to show full abstract
Abstract A new nanopore array/Au electrode was fabricated and applied to the detection of microRNA. The electrode was prepared via alternating current electrodeposition of Au nanowires in the nanopores of a porous anodic alumina membrane (without the need to remove aluminum or any barrier layer). The internal surface of the nanopores was then functionalized with an electrically neutral morpholino with a specially designed sequence. The resulting electrode shows excellent sensitivity and selectivity in detection of the target miRNA-155. The detection limit is 10 aM and the linear range 10 aM–1 nM in aqueous solution and the electrode can be reused in 6 M urea solution. In addition, it shows good linearity (100 aM–0.1 nM) in a serum sample. This new biosensor is expected to enhance miRNA detection in clinical diagnosis.
               
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