LAUSR

Alkaline pretreatment shows a good effect on delignification of lignocellulosic biomass, and carbide slag, an alkaline industrial waste, was first applied to pretreat turfgrass pruning. Three thermo-alkaline pretreatment methods were compared to evaluate their ability to destroy the biomass structure and to enhance the enzymatic hydrolysis efficiency of turfgrass pruning, namely thermo-NaOH pretreatment, thermo-lime pretreatment, and thermo-carbide slag pretreatment. Results showed that the maximum net reducing sugar yield of 269.55 mg/g raw biomass was achieved by thermo-carbide slag pretreatment. Compared with raw biomass, the lignin and hemicellulose content of turfgrass pruning after thermo-carbide slag pretreatment reduced by 35.57% and 62.40%, respectively. SEM and FTIR analyses showed that the surface structure and chemical groups of turfgrass pruning were obviously destroyed by thermo-carbide slag pretreatment. There was a high positive correlation between the enzymatic hydrolysis efficiency and cellulose content of substrate. However, a high negative correlation was observed between the enzymatic hydrolysis efficiency and lignin content of substrate. The enzymatic hydrolysis kinetics with different pretreatments were well described by the fractal-like kinetics model, and the rate constant of enzymatic hydrolysis of thermo-alkali pretreated turfgrass pruning was about three times of that of the unpretreated turfgrass pruning. Overall, carbide slag is an ideal pretreatment material for enzymatic hydrolysis of turfgrass pruning in terms of hydrolysis efficiency and waste reuse.