LAUSR

ABSTRACT The bioaccessibility of environmental contaminants has been assessed widely using in vitro simulation; however, the physiological parameters used vary greatly. In this study, we assessed the influence of various physiological parameters and food material on the oral or inhalation bioaccessibility of PM2.5‐bound hydrophobic organic contaminants (HOCs), including halogenated flame retardants (HFRs), organophosphorus flame retardants (OPFRs), and polycyclic aromatic hydrocarbons (PAHs). The results showed that physiologically based pepsin and pancreatin have a small influence on the HOC liberation from particles. The bioaccessibility increased dramatically when the bile salt concentrations exceeding the critical micelle concentration, and application of porcine bile salts probably lead to underestimated bioaccessibility. Protein and carbohydrates significantly increased the bioaccessibility of most HOCs, while a significant bioaccessibility reduction was caused by green tea. The bioaccessibility of most HOCs was not promoted by liquor under normal physiological condition, but was significantly promoted under fast condition. Long residence time of PM2.5 in the lung (15 days) would result in higher mobilization of PAHs into the lung fluid than short time (one day). However, the inverse time‐dependence for OPFRs suggests degradation in the lung fluid. A mechanism of hydrolysis of organophosphorus ester is hypothesized, and the half lives ranged from 17 to 90 days. HighlightsPepsin and pancreatin have a small influence on the oral HOC bioaccessibility.Bioaccessibility increases dramatically when bile salt concentrations exceed CMC.Green tea leads to a significant reduction in the oral bioaccessibility of HOC.Bioaccessibility was not significantly promoted by liquor under normal condition.Degradation of OPFRs in simulated lung fluid was found with half‐lives of 17–90 d.