LAUSR

The nitrous oxide (N2O) flux and its possible production pathways from stormwater biofilters in response to moisture content (MC) due to a shift from dry to wet weather was investigated. In this study, we evaluated changes in the composition of the bacterial community, the relative abundance of functional genes, and N2O emission rate in laboratory-scale stormwater biofilters in response to changes in MC. The results indicated that N2O flux correlated positively with soil MC (r=0.722 p<0.01). We observed a higher rates of N2O flux when shifting from dry to wet conditions; Notably, these values decreased substantially within 8-24 hours in response to the rapid decline in MC, and then gradually decreased and stabilized at 4.4-12.0 μg/m2·h. The relative abundance of ammonia-oxidizing and denitrifying bacteria, as well as the relative abundance of functional groups associated with denitrification was higher under conditions of low soil MC than in those with high MC. Furthermore, the abundance of bacterial genes norB (r=0.716 p<0.01) and hao (r=0.917 p<0.01) was associated with higher N2O emission in soils with high MC. Studies with the stable isotope (15N) revealed that 15N enrichment in N2O was primarily via denitrification pathways and labeled ammonium ion (15NH4+). Taken together, our results suggested that nitrifier denitrification is the main pathway generating N2O emission in soils with high MC, which may be caused by the high molar ratio of NH3 to total nitrogen in the influent.