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Highlights • Identified over 90 putative polysaccharide degrading ORFs in C. fimi genome.• Cloned 14 putative cellulolytic ORFs as BioBricks, screened them for activity.• Partially purified AfsB, BxyF, BxyH and… Click to show full abstract
Highlights • Identified over 90 putative polysaccharide degrading ORFs in C. fimi genome.• Cloned 14 putative cellulolytic ORFs as BioBricks, screened them for activity.• Partially purified AfsB, BxyF, BxyH and XynF and characterised them further.• BxyH proved highly temperature and alkaline pH tolerant.• BioBricks are an easy method for screening genes for specific activities.
Keywords:
genome cellulomonas;
characterisation novel;
enzymes genome;
biomass degradation;
novel biomass;
degradation enzymes
Journal Title: Enzyme and Microbial Technology
Year Published: 2018
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Journal Title: Enzyme and Microbial Technology
Year Published: 2018
Link to full text (if available)
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recommendations!