Photo by nci from unsplash
Abstract We developed Ni2+-nitrilotriacetic acid (NTA) functionalized polystyrene (Ni2+-NTA-PS) nanoparticles encapsulated with magnetic Fe3O4 nanoparticles (Fe3O4@Ni2+-NTA-PS nanoparticles) via miniemulsion/solvent evaporation method for the affinity purification of his6-tag recombinant proteins. Fe3O4@Ni2+-NTA-PS… Click to show full abstract
Abstract We developed Ni2+-nitrilotriacetic acid (NTA) functionalized polystyrene (Ni2+-NTA-PS) nanoparticles encapsulated with magnetic Fe3O4 nanoparticles (Fe3O4@Ni2+-NTA-PS nanoparticles) via miniemulsion/solvent evaporation method for the affinity purification of his6-tag recombinant proteins. Fe3O4@Ni2+-NTA-PS nanoparticles have a high surface to volume ratio, which enhanced the protein immobilization efficiency through the specific interaction between his6-tag of protein and Ni2+-NTA end of the polymer. The potential of Fe3O4@Ni2+-NTA-PS nanoparticles is demonstrated by a direct comparison with commercially available Ni2+-NTA-magnetic beads in his6-protein immobilization and recovery. Furthermore, the practical application of Fe3O4@Ni2+-NTA-PS magnetic nanoparticles is illustrated in his6-protein separation from crude cell lysate.
Journal Title: European Polymer Journal
Year Published: 2019
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!