LAUSR

Abstract Natural polymers have shown special advantages in application as drug delivery carriers. However, microbial e-polylysine seems to have been snubbed. In this study, we constructed a drug delivery system based on natural e-polylysine. Amphipathic graft copolymer, methoxypoly(ethylene glycol)-graft-e-polylysine-tetraphenylethylene (mPEG-g-Plys-TPE), was synthesized based on microbial e-polylysine which was modified completely with PEG and TPE. MPEG-g-Plys-TPE could self-assemble into nanomicelles with stable shell-core structure. PEG served as the hydrophilic shell to provide long blood circulation and biocompatibility. TPE formed the hydrophobic core with aggregation-induced emission (AIE) effect to load drugs. The physical and chemical properties, including size distribution, morphology, stability and photoluminescent effect, were investigated in detail. In vitro drug release indicated pH-dependent patterns. Furthermore, cytotoxicity of doxorubicin (DOX)-loaded micelles against HT-29, HCT116 and SW480 was significantly higher than that of free DOX, which was explained by the mechanism of cellular uptake against tumor cells. In summary, all results suggested that mPEG-g-Plys-TPE as new type biomaterials exhibited great potential for drug delivery.