LAUSR

Abstract The present study focused on the biochemical evaluation of artichoke extract, furthermore, in vitro and in silico evaluation its potential for inhibition of acetylcholinesterase activity. The total phenolic, flavonoids, triterpenoids, tannins, and sulfide polysaccharides content were 193.63, 0.038, 71.43, 13.49, and 115.612 μg/g. (B 2) content was 60.95 μg/g, (B 9) was 6.85 μg/g, retinol (A) was 0.248422 μg/g, and (B 12) was 6.07 μg/g. Benzoic acid, ellagic acid, and caffeine were 589.91, 573.07, and 382.03 μg/100g. IC 50 values of ellagic acid, benzoic acid, caffeine, extract, and donepezil had of 16.97, 26.0, 27.28, 31.04, and 133 μg/mL. In In vitro assay; caffeine exhibited a significant noncompetitive inhibition of acetylcholinesterase (AChE) with IC 50 of 1.013 mg/mL. ellagic acid and extract exhibited competitive inhibition with IC50 of 1.927 and 5.705 mg/mL. Donepezil exhibited mixed modes of inhibition with IC50 of 0.0034 mg/mL, but benzoic acid did not exhibit any anti-AChE. In silico molecular docking of human AChE (4EY7) with donepezil, caffeine, ellagic acid, and acetylcholine showed binding energy ΔG values of −9.47, −6.07, −9.39, and −5.69 Kcal/mol, respectively. Accordingly, the current study emphasized significant of antioxidant and anti-acetylcholinesterase potential of artichoke extract and related phenolic compounds which might take the attention of scientists to explore a neuroprotective effect of artichoke extract and its promising effect towards Alzheimer disease.