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MATERIALS AND METHODS: Embryos were cultured in the time-lapse incubator Geri_ (Genea Biomedx, Australia) with 6 patient-individual chambers. 3 chambers worked under humid conditions (HC) and 3 under dry conditions… Click to show full abstract
MATERIALS AND METHODS: Embryos were cultured in the time-lapse incubator Geri_ (Genea Biomedx, Australia) with 6 patient-individual chambers. 3 chambers worked under humid conditions (HC) and 3 under dry conditions (DC), 83 and 93 patients respectively. Geri dishes with 80 mL of culture media were covered with an oil overlay, and cultured for 5-6 days. The effects of humidity were assessed retrospectively with regard to blastocyst, pregnancy and miscarriage rates. Its influence over morphokinetic parameters was evaluated by using the time-lapse system. Additionally, a preliminary study regarding the oxidative status of the spent embryo culture media was conducted using the TCL (Thermochemiluminescence) Analyzer TM.
Journal Title: Fertility and Sterility
Year Published: 2018
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