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Isolation and characterization of spontaneous phage-resistant mutants of Lactobacillus paracasei

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Abstract Spontaneous phage-resistant mutants were isolated from Lactobacillus paracasei LPC by agar plate (AP) and secondary-culture (SC) methods. They were characterized by cell and colony morphologies, carbohydrate fermentation patterns, phage… Click to show full abstract

Abstract Spontaneous phage-resistant mutants were isolated from Lactobacillus paracasei LPC by agar plate (AP) and secondary-culture (SC) methods. They were characterized by cell and colony morphologies, carbohydrate fermentation patterns, phage resistance stability, efficiency of plaquing (EOP), acidifying and milk acidification kinetics. Only 98 out of 175 isolates (56%) proved to be true phage-resistant mutants and the SC method was more efficient than the AP method. Although the phage resistance stability varied among the mutants isolated, the EOP values were mostly very high (

Keywords: spontaneous phage; resistant mutants; isolation characterization; phage; phage resistant; lactobacillus paracasei

Journal Title: Food Control
Year Published: 2019

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