LAUSR

Abstract This study aimed to investigate aflatoxin M1 (AFM1) contamination in pasteurized, extended shelf life (ESL) and ultra-high temperature (UHT) milk in central China in winter and summer, and to assess the risk of exposure to milk AFM1 in 10 different age groups. A total of 547 milk samples were collected in Henan, Hubei and Hunan provinces from 2019 to 2020, of which 142 pasteurized milk samples, 68 ESL milk samples and 69 UHT milk samples were collected in summer, and 161 pasteurized milk samples, 79 ESL milk samples and 28 UHT milk samples were collected in winter. The concentrations of AFM1 in these samples were determined by ELISA, with a recovery and relative standard deviation of 95.0–98.6% and 3.31–4.18%, respectively. AFM1 was detected in 431 samples (78.8%) with an average concentration of 19.6 ng/L in positive samples. The AFM1 concentrations in 29 samples (5.3%) were higher than the maximum residue level (MRL) of 50 ng/L set by the EU, but much lower than the value of 500 ng/L set by China. The average concentration of AFM1 (25.4 ng/L) in winter milk was 88.1% higher (p