LAUSR

PURPOSE The purpose of the present study was to investigate sex-related differences in the stiffness of the Achilles tendon and gastrocnemius muscle at rest and tensioned state. Another purpose of the study was to investigate the inter-observer reliability and inter-day repeatability of MyotonPRO, a portable myotonometer, in measuring tendon and muscle stiffness. METHODS The study included 73 healthy participants (19 males for the reliability and repeatability study; 24 males and 30 females for the experimental study) with an age range of 19-27 years. The stiffness of the Achilles tendon and medial gastrocnemius muscle was measured with a portable myotonometer (MyotonPRO). The stiffness measurements of the Achilles tendon and medial gastrocnemius muscle were performed at 0° and 10° of ankle joint dorsiflexion. The stiffness measurements were performed by two physiotherapists to determine the inter-observer reliability of the device. For the inter-day repeatability (or between-day precision), the same individuals were reassessed by the same examiner after a 3-day interval. RESULTS It was found that MyotonPRO has excellent inter-observer reliability and inter-day repeatability in measuring the stiffness of the Achilles tendon and medial gastrocnemius muscle (ICC=0.83-0.98). The stiffness of the Achilles tendon and gastrocnemius muscle at neutral and 10° joint dorsiflexion was higher in males compared to females (p<0.05); however, the stiffness value of difference between neutral and 10° joint dorsiflexion for the Achilles tendon and gastrocnemius muscle was similar in both groups (p>0.05). DISCUSSION The obtained results suggest that MyotonPRO is a reliable and repeatable device in measuring the stiffness of the Achilles tendon and gastrocnemius muscle. Furthermore, males have stiffer Achilles tendon and gastrocnemius muscle, compared to females; however, the change in the stiffness of the assessed tissues caused by joint motion was similar in both sexes.