LAUSR

The secondary screwworm, Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae), is a carrion-breeding species of veterinary, medical, and forensic importance. It is very abundant in the Nearctic and Neotropical regions and is one of the most common colonizers of vertebrate remains in the southern United States. Therefore, it is of great evidential value in estimating the minimum time of colonization (TOC) of remains related to forensic investigations. So far, studies have investigated the effects of several biotic and abiotic factors on C. macellaria. However, no data on the specific impact of food source moisture on the larval development of this species are known to have been published. In this study, the effects of diet moisture on larval development time, larval length, and weight over time, as well as adult emergence and weight were investigated. C. macellaria was reared on diets prepared from freeze-dried bovine liver with varying moisture content (0.0, 33.0, 50.0, or 70.8%) at 25.6 °C, 77% RH, and 14:10 L:D. Frozen-thawed liver was used as a control. Water content was found to significantly impact immature development time and corresponding life-history traits, both within and among developmental stages of C. macellaria. This result indicates moisture content of the carrion source should be considered when estimating time of colonization and identification of immature stage of flies in forensic investigations. Furthermore, as diet moisture content significantly affected the dry mass of emerging adults, the mass of adults collected at a crime scene could provide useful information as it could be an indicator of the condition the remains were in during time of colonization, including the moisture content of the tissue.