LAUSR

Abstract Although critical in phagocytosis in innate immunity, reactive oxygen species (ROS) collaterally inflict damage to host phagocytes because they indiscriminate targets. Since Nrf2 increases the expression of anti‐oxidant enzymes that nullifies ROS, ROS activating Nrf2 is a critical negative regulatory step for countering the deleterious effects of ROS. Here, we postulate whether, along with ROS activating Nrf2, NADPH oxidase components also participate in direct activation of Nrf2, contributing to protection from ROS. Our results show that the p47phox of the NADPH oxidase, but not p65phox or p40phox, physically binds to Nrf2, activating the Nrf2 function. p47phox binding to Nrf2/Keap1 complex suppresses the ubiquitination of Nrf2, while p47phox becomes ubiquitinated by Keap1. p47phox increases the nuclear translocation of Nrf2 and the expression of Nrf2‐dependent genes, whereas genetic ablation of p47phox decreases the expression of those genes. In a lipopolysaccharide‐induced acute lung inflammation mouse model, selective expression of p47phox in mouse lungs induces the expression of Nrf2‐dependent genes and is sufficient to suppress neutrophilic lung inflammation. Therefore, our findings suggest that p47phox is a novel regulator of Nrf2 function. Graphical abstract No caption available. Highlightsp47phox of NADPH oxidase binds to Nrf2, without disrupting Nrf2/Keap1 complex.p47phox binding to Nrf2 results in the ubiquitination of p47phox by Keap1.p47phox suppresses the ubiquitination of Nrf2.p47phox induces the expression of Nrf2‐dependent genes in cells and mouse lungs.p47phox suppresses inflammation in a LPS‐induced lung inflammation mouse model.