LAUSR

Abstract Massively parallel sequencing technology has been demonstrated as a potential tool for genetic studies and recently, for forensic context. ForenSeq™ DNA Signature Prep Kit (Illumina, Inc.) has been designed exclusively for forensic DNA analysis that autosomal STRs, X-STR, Y-STR and identity SNPs can be sequenced simultaneously. These informative genetic data empower the achievement of global DNA database presenting in autosomal STRs and Y-STR. The Y-STRs comprised of 8 core loci, 2 multi-copy loci and other 14 Y-STR loci. Here, we examined 24 Y-STR of 182 Thai male population that originate from Central, Western and Southern regions of Thailand. In this study, the average gene diversity (GD) of this kit is 0.7031. The lowest gene diversity was 0.4187 from DYS391 locus and DYS385a-b showed the highest gene diversity (0.9582). One hundred and seventy-two haplotypes were observed and therefore, these 24 Y-STR markers would be beneficial for forensic investigation, paternity test and genealogy study in Thai population.