LAUSR

The role of nitrogen (N) fixation in determining the frequency, magnitude, and extent of harmful algal blooms (HABs) has not been well studied. Dolichospermum is a common HAB species that is diazotrophic (capable of N fixation) and thus growth is often considered never to be limited by low combined N sources. However, N fixation is energetically expensive and its cost during bloom formation has not been quantified. Additionally, it is unknown how acclimation to differing nutrient ratios affects growth and cellular carbon (C):N stoichiometry. Here, we test the hypotheses that diazotrophic cyanobacteria are homeostatic for N because of their ability to fix atmospheric N2 and that previous acclimation to low N environments will result in more fixed N and lower C:N stoichiometry. Briefly, cultures that varied in resource N:phosphorus (P) ranging from 0.01 to 100 (atom), were seeded with Dolichospermum which were previously acclimated to low and high N:P conditions and then sampled temporally for growth and C:N stoichiometry. We found that Dolichospermum was not homeostatic for N and displayed classic signs of N limitation and elevated C:N stoichiometry, highlighting the necessary growth trade-off within cells when expending energy to fix N. Acclimation to N limited conditions caused differences in both C:N and fixed N at various time points in the experiment. These results highlight the importance of environmentally available N to a diazotrophic bloom, as well as how previous growth conditions can influence population growth during blooms experiencing variable N:P.