LAUSR

Abstract Increases in general circulatory time, half-life and bioavailability increase the efficacy of any drug molecule. Rutin (3, 3′, 4′, 5, 7 -pentahydroxyflavone-3-rhamnoglucoside) is a flavonoid known for its ability to regulate cell metabolism, gene expression, and protect against oxidative stress, although certain biophysical aspects of their functioning are not yet clear. Non protein and small protein molecules with electrophilic properties are transported through the blood to their target site by binding with albumin. The authors therefore hypothesized that modification of rutin-albumin binding by changing the microenvironment in which it is delivered can increase the efficacy of the molecule. Therefore, the interaction of rutin in different solvent systems with albumin have been studied to evaluate the bioavailability and efficacy of the molecule to introduce it as a drug for combating UVB radiation-induced damage in biological systems. We evaluated the effect of rutin–albumin interaction in blood plasma in various modes of delivery (in DMSO, alcohol and PEG). The biological activity and efficacy of different solvents for rutin delivery and its bioavailability were studied using UVB irradiated human peripheral blood mononuclear cells in vitro. Amelioration of UVB radiation-induced oxidative damage and cell death by rutin has been studied by MTT assay and Tryphan blue exclusion assay and LDH-cytotoxicity assay. In these three studied systems the most effective concentration of rutin was also determined.