LAUSR

Abstract High hydrostatic pressure (HHP) and centrifugation were combined to produce a 5-methyltetrahydrofolate (5-MTHF)-enriched extract from egg yolk. This study focused on the effect of HHP treatment (200–600 MPa; 5, 10 min) in extraction of 5-MTHF from egg yolk and granule. Specifically, HHP-treatment at 400 MPa for 5 min significantly (p  Industrial relevance: HHP combined with centrifuge separation was used an efficient and eco-friendly process for improving the recovery of water soluble vitamin 5-MTHF extracted from egg yolk granule. HHP treatment at 400 MPa for 5 min was the optimum condition where a higher concentration of 5-MTHF separated from granule. This study shows promising results in relation to applications of HHP in improving extraction and recovery of bioactive components from egg yolk and the future applications of granules as an ingredient in the food industry.