LAUSR

Vaginal dysbiosis is characterized by the disturbed vaginal microbiota and associated with various gynecological diseases, and it is an urgent need for the development of effective agent due to its high recurrence. In the present study, the vaginal dysbiosis model was developed to study the positive effect of the vaginal microbiota transplantation (VMT) or probiotic combination (PC, containing Lactobacillus helveticus, L. crispatus, L. acidophilus, L. gasseri, and L. salivarius) on vaginal dysbiosis. Our results indicated that VMT or PC significantly reduced the introducing bacteria-induced inflammation (infiltrations of neutrophils, lymphocytes, and monocytes) in uterine wall, the enrichment of the proinflammatory cytokines [interleukin (IL)-1β and tumour necrosis factor (TNF)-α] in vaginal tissue, and restored the disturbed vaginal microbiota to normal level (increasing numbers of Lactobacillus and decreasing numbers of Enterobacter and Enterococcus), by which it should be beneficial for avoiding the recurrence of vaginal dysbiosis. Therefore, VMT or PC might be an effective agent for the treatment of the bacterial-induced vaginosis.