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Purification and characterization of exopolysaccharide produced by Weissella cibaria YB-1 from pickle Chinese cabbage.

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An exopolysaccharide (EPS) was produced by Weissella cibaria YB-1 isolated from pickle Chinese cabbage. The EPS was purified and characterized. The monosaccharide composition of the EPS was glucose, and its… Click to show full abstract

An exopolysaccharide (EPS) was produced by Weissella cibaria YB-1 isolated from pickle Chinese cabbage. The EPS was purified and characterized. The monosaccharide composition of the EPS was glucose, and its molecular mass was 3.89 × 106 Da, as determined by gas chromatography (GC) and high performance liquid chromatography (HPLC). The structural characterization of purified EPS determined by Fourier transform infrared (FT-IR) spectra and nuclear magnetic resonance (NMR) spectra demonstrated that W. cibaria YB-1 synthesized a linear dextran that predominately had α-(1 → 6) glycosidic linkages with only a few α-(1 → 3) (4.3%) linked branches. The water solubility index (WSI), water holding capacity (WHC) and emulsifying activity (EA) of YB-1 dextran were 95.23 ± 4.45, 287.84 ± 16.23 and 84.43 ± 3.65%, respectively. The in-vitro antioxidant activities of the dextran showed good scavenging effects on superoxide anion radical and hydroxyl radical.

Keywords: chinese cabbage; weissella cibaria; pickle chinese; produced weissella; characterization

Journal Title: International journal of biological macromolecules
Year Published: 2018

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