LAUSR

Biocompatible nanocomposite films based on chitosan (CS) and polyethylene glycol (PEG) polymers containing cephalexin (CFX) antibiotic drug and zeolitic imidazolate framework-8 (ZIF-8) nanoparticles (NPs) were designed and fabricated to develop wound dressing materials capable of controlled drug release. Swelling experiment was performed in three acidic, neutral, and alkaline solutions. The tensile strength test reflected that upon increasing the NPs loading within the films, the tensile strength was enhanced but the elongation at break was diminished. The release of the CFX was intensively increased within approximately 3, 8, and 10 h (burst release) in acidic, neutral, and alkaline media, respectively while after that the CFX was smoothly released over time (sustained release). The antibacterial activities of all films were examined against Gram-positive (S. aureus, B. cereus) and Gram-negative (E. coli, P. aeruginosa, and Acinetobacter) bacteria frequently found in the infected wounds. Moreover, the MTT assay revealed that all films had high cell viabilities towards the L929 fibroblast cells confirming these nanocomposites could be used as favorable wound dressing materials. Finally, the film containing 4% ZIF-8 NPs (film 5) was chosen as the best sample due to it revealed appropriate mechanical properties, swelling, drug release and cell viability among all samples examined.