LAUSR

INTRODUCTION Smoking is an independent cause of cervical cancer, which is the 4th most common malignancy in women. It is currently not known if tobacco consumption causes chromosomal damage (which is a hallmark of human cancer) in cervical cells and if age and the hormonal status have an impact on tobacco induced genetic instability in the cervix. METHODS We conducted a study with pre- and post-menopausal women smokers and never-smokers (25/group). Smokers consumed 30 light/medium cigarettes/day and were matched with the non-smoking group. Cervical cells were analyzed for induction of micronuclei (MN) which are caused by structural/numerical chromosomal aberrations; additionally, other nuclear anomalies reflecting genomic instability and cytotoxicity were scored. Furthermore, the frequencies of basal cells were recorded which reflect the mitotic activity of the mucosa. RESULTS MN and other abnormalities were increased in both groups of smokers. The effects were most pronounced in postmenopausal smokers (i.e. 2-fold higher) compared to premenopausal smokers. Also the number of basal cells (indicative for cell proliferation) was clearly enhanced in older women. Tar and nicotine had no detectable impact on chromosomal damage but a clear association with pack-years was observed. CONCLUSIONS Smoking increased chromosomal instability, cytotoxicity and induced cell divisions in cervical mucosa cells of pre- and post-menopausal women. The effects were more pronounced in the latter group indicating a higher risk for diseases (including cancer) that are causally related to DNA damage.