LAUSR

Abstract A novel integral imaging microscope system with high magnification is analyzed and implemented in this paper. In our system, we use an inverted fluorescence microscope for specimens amplification, and apply a 3-D information acquisition system for 3-D information acquisition, and utilize an EMCCD for acquiring elemental images arrays formed by a microlens array and relay system. By using our experimental prototype, we record 12 × 12 elemental images arrays of different type specimens successfully, and generate depth slice images by CIIR method. Comparing with a conventional light field microscope, the magnification of our system is improved hundreds times because of remaining eyepiece and designing novel experimental configuration, then we can acquire more fine 3-D information of the specimen. Moreover, we develop a diffuse transmission element for acquiring 3-D information which is carried by scattered light, so we can acquire more plentiful 3-D information. Finally, we also demonstrate the validity and the effectiveness of our high magnification system by biological microorganism experimental data.