LAUSR

Graphical abstract Figure. No Caption available. Abstract The self‐assembly process of amphiphilic molecules into solvents results in different mesophases, such as inverse cubic and hexagonal that both belong to the wider category of lyotropic liquid crystals. The above mesophases can be further exploited upon the formation of liquid crystalline nanoparticles, cubosomes and hexosomes respectively, which may be utilized as drug delivery nanosystems, exhibiting major advantages. In the present study, liquid crystalline nanoparticles were prepared and evaluated in terms of morphology and physicochemical behavior. The goal of this study is to examine the effect of the different formulation parameters, as well as the effect of the different microenvironmental factors (temperature, ionic strength, pH, serum proteins presence) on their behavior. The physicochemical behavior and the morphology of the systems were investigated by X‐Ray Diffraction (XRD), cryogenic‐Transmission Electron Microscopy (cryo‐TEM), fluorescence spectroscopy and a gamut of light scattering techniques. The formulation process was proved to influence strictly the physicochemical behavior of the prepared nanosystems. They presented colloidal stability over time and upon ionic strength increase, but they were affected by the presence of proteins and presented reversible structure alterations upon temperature increase. Their morphological structure and internal microenvironment, reflected by micropolarity and microfluidity, were also influenced by the formulation parameters.