LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Improved histidinylated lPEI polyplexes for skeletal muscle cells transfection

&NA; Linear Polyethylenimine (lPEI) is an efficient cationic polymer for transfecting cells, both in vitro and in vivo, but poses concerns regarding cytotoxicity. Histidinylated lPEI (His‐lPEI) exhibits also high transfection… Click to show full abstract

&NA; Linear Polyethylenimine (lPEI) is an efficient cationic polymer for transfecting cells, both in vitro and in vivo, but poses concerns regarding cytotoxicity. Histidinylated lPEI (His‐lPEI) exhibits also high transfection efficiency but lower cytotoxicity than lPEI. For the first time, we tested polyfection efficiency of polyplexes comprising both lPEI and His‐lPEI. A series of pDNA polyplexes was prepared with mixtures of lPEI and His‐lPEI and the amount of each polymer within His‐lPEI/lPEI polyplexes was determined by flow cytometry. We show that His‐lPEI/lPEI polyplexes exhibit properties similar to lPEI polyplexes in terms of size, morphology, assembly with pDNA, and polyplex stability while His‐lPEI/lPEI polyplexes exhibit properties similar to His‐lPEI polyplexes in terms of buffering capacity. Compared to polyplexes consisting only of lPEI or His‐lPEI, the transfection profile reveals that His‐lPEI/lPEI polyplexes containing 30% to 57% lPEI strongly increase polyfection efficiency of NIH3T3 fibroblasts and murine, as well as human skeletal muscle cell lines without cytotoxicity. Importantly, improved transfection of human dystrophin deficient skeletal muscle cell lines was obtained. These results indicate that His‐lPEI/lPEI polyplexes are an improved non‐viral vector for efficient transfection of dystrophin deficient skeletal muscle cell lines that should be tested on animals. Graphical abstract Figure. No caption available.

Keywords: lpei; skeletal muscle; transfection; lpei polyplexes; lpei lpei

Journal Title: International Journal of Pharmaceutics
Year Published: 2019

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.