Abstract Defatted Schizochytrium residue was used as a supplementary feedstock for efficiency erythritol production in this study. Microalgae are attractive feedstocks for the microbial process. However, proper enzymatic hydrolysis and… Click to show full abstract
Abstract Defatted Schizochytrium residue was used as a supplementary feedstock for efficiency erythritol production in this study. Microalgae are attractive feedstocks for the microbial process. However, proper enzymatic hydrolysis and toxicity alleviation must be considered for efficient fermentation. A strategy was proposed herein for producing erythritol from enzymatic hydrolysate of the defatted Schizochytrium residue (SRH), resorting to the wheat (Triticum aestivum L.) straw biochar-incorporated solid-state fermentation (SSF) of agricultural wastes to relieve the toxicant inhibition. Enhanced erythritol production (190.5 mg/gds) was achieved in batch SSF supplemented with 4% biochar and 20 % concentrated SRH, which was obtained from the cellulose–protease (1:1) system performed under pH 5.0 and 45 °C for 24 h with 0.8 % (m/v) of enzyme addition. Fed- and repeated-batch fermentation demonstrated enduring high cell viability and improved erythritol yield of 223.2 and 205.4 mg/gds, respectively. Cost analysis suggested high availabilities of raw substrates for erythritol cultivation, which was energy conserving and operable. Moreover, SRH was proven to be an economic alternative feedstock for low-cost erythritol production via the biochar-incorporated SSF.
               
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