LAUSR

OBJECTIVE MicroRNAs (miRNAs) have engaged in the progression of myocardial infarction (MI). Nevertheless, the mechanism of miR-150-5p in MI is still in its infancy. Therefore, the present study was set out to investigate the effect of bone marrow mesenchymal stem cells derived exosomes (BMSCs-Exo) and miR-150-5p in MI via regulating B-cell lymphoma-associated X (Bax). METHODS BMSCs-Exo were isolated and extracted, and exosomes with miR-150-5p agomir or antagomir was constructed. Then, a mouse MI model was induced by ligation of left anterior descending coronary artery. Mice were injected with exosomes and miR-150-5p agomir/antagomir to detect cardiac function, pathological changes, and apoptosis rate of cardiomyocytes. miR-150-5p and Bax expression in myocardial tissues were tested. The targeting relationship between miR-150-5p and Bax was verified. RESULTS BMSCs and exosomes were successfully extracted. BMSCs-derived exosomal miR-150-5p improved cardiac function, alleviated pathological changes of myocardium, decreased apoptosis rate of cardiomyocytes in MI mice. miR-150-5p expression was reduced and Bax expression was elevated in myocardial tissues of MI mice, while exosomes raised miR-150-5p expression and reduced Bax expression in MI mice. miR-150-5p was found to target Bax. CONCLUSION On all accounts, the present study provides evidence that BMSCs-derived exosomal miR-150-5p attenuates apoptosis of cardiomyocytes and improves cardiac function of MI mice via targeting Bax.