LAUSR

Abstract Passive samplers are needed to collect bioaerosols over broad spatiotemporal scales. Here, we present the design and development process of a novel, passive bioaerosol sampler using polarized, ferroelectric polymer film (poly(vinylidene fluoride), PVDF). First, the elution efficiencies of spiked bacteria and fungal spores from PVDF, prototype film holder materials (3D-printed plastics), and controls (PTFE filters and electrostatic dust cloths) were investigated. Second, a wind tunnel was used to determine optimal air channel widths between parallel sheets of PVDF for efficient collection of charge neutralized and non-charge neutralized test particles in viral, bacterial and fungal size ranges flowing with typical indoor air velocities. Particle collection efficiencies were then compared for PVDF, a PVDF copolymer (77% PVDF, 23% TrFE) and control materials (polyvinylchloride and polyfluoroalkoxy alkane). Third, a calm air settling chamber was used for proof of concept tests. Spiked microorganisms were removed with 100% elution efficiency from the PVDF and 3D-print materials. PVDF gave significantly greater collection efficiency (~30%) when using air channel widths of 2.25 mm compared to other tested widths (p