LAUSR.org creates dashboard-style pages of related content for over 1.5 million academic articles. Sign Up to like articles & get recommendations!

Development of zinc oxide-based sub-micro pillar arrays for on-site capture and DNA detection of foodborne pathogen.

Photo from wikipedia

Prevention and early detection of bacterial infection caused by foodborne pathogens are the most important task to human society. Although currently available diagnostic technologies have been developed and designed for… Click to show full abstract

Prevention and early detection of bacterial infection caused by foodborne pathogens are the most important task to human society. Although currently available diagnostic technologies have been developed and designed for detection of specific pathogens, suitable capturing tools for the pathogens are rarely studied. In this paper, a new methodology is developed and proposed to realize effective capturing through touchable flexible zinc oxide-based sub-micro pillar arrays through genetic analysis. Zinc oxide coated pillar arrays have a high surface area, flexible, and adheres strongly to bacteria. Therefore, it contributes to enhance the bacterial capturability. An in-depth analysis on the sub-sequential capturing process at the bacterial cell-pillar interface is presented. By carefully observing the structural changes and performing numerical analysis under different reaction times, the results are presented. The resulting zinc oxide coated pillar arrays exhibited comprehensive capturability. These pillars were able to detect pathogenic bacteria due to a combination of complex structures, depletion force, and high surface electrostatics. The developed sub-micro pillars successfully captured and detected infectious foodborne bacteria of Escherichia coli in the range of 106-101 CFU/mL.

Keywords: sub micro; detection; pillar arrays; zinc oxide

Journal Title: Journal of colloid and interface science
Year Published: 2019

Link to full text (if available)


Share on Social Media:                               Sign Up to like & get
recommendations!

Related content

More Information              News              Social Media              Video              Recommended



                Click one of the above tabs to view related content.